One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles

2001 ◽  
Vol 22 (14) ◽  
pp. 2872-2880 ◽  
Author(s):  
Kimie Murayama ◽  
Tsutomu Fujimura ◽  
Masataka Morita ◽  
Noriko Shindo
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Density Gradient ◽  
Rat Liver ◽  
Liver Tissue ◽  
Sodium Dodecyl ◽  
Subcellular Fractionation ◽  
Two Dimensional ◽  
Main Fraction ◽  
Dodecyl Sulfate ◽  
One Step

A small molecular, a big scissoring effect: sodium dodecyl sulfate working on two-dimensional metal organic frameworks

CrystEngComm ◽  
2021 ◽  
Author(s):  
Qing Luo ◽  
Zhen Ding ◽  
Huamin Sun ◽  
Zhen Cheng ◽  
Naien SHI ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Active Sites ◽  
Metal Organic Framework ◽  
Metal Organic Frameworks ◽  
Sodium Dodecyl ◽  
Advanced Materials ◽  
Controlled Synthesis ◽  
Two Dimensional ◽  
Dodecyl Sulfate ◽  
Metal Organic

Ultrathin two-dimensional (2D) metal-organic framework (MOF) nanosheets are prosperous advanced materials due to their particularly thin thickness and exposed active sites. The difficulty in the controlled synthesis of 2D MOF...

Two-dimensional electrophoretic analysis of cytosols from human breast tumors: optimal migration conditions.

1984 ◽  
Vol 30 (12) ◽  
pp. 1947-1949 ◽  
Author(s):  
P Motté ◽  
J M Bidart ◽  
J C Delarue ◽  
E Comoy ◽  
P Moingeon ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Breast Tumors ◽  
Electrophoretic Analysis ◽  
Ph Gradient ◽  
Two Dimensional ◽  
Human Breast ◽  
Cytosolic Proteins ◽  
Optimal Migration ◽  
Dodecyl Sulfate

Abstract In an examination of cytosols from human breast tumors, we performed two-dimensional electrophoretic analysis. Several migration conditions were tried in a search for a homogeneous repartition of cytosolic proteins. The most workable gels were obtained with a 4.40 to 8.05 pH gradient in the first dimension and a homogeneous 125 g/L acrylamide/sodium dodecyl sulfate gel, 1 mm in thickness, in the second dimension. The practicability of this method is discussed.

A simple method of eluting proteins from two-dimensional gels.

1982 ◽  
Vol 28 (4) ◽  
pp. 756-758 ◽  
Author(s):  
J Guevara ◽  
E A Chiocca ◽  
F C Clayton ◽  
A C von Eschenbach ◽  
J J Edwards
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Phosphate Buffer ◽  
Sodium Dodecyl ◽  
Two Dimensional ◽  
Simple Method ◽  
Dodecyl Sulfate

Abstract Apo A-I lipoprotein was extracted from the appropriate pieces of two-dimensional gel patterns, by electrophoresis onto hydroxylapatite overlaying a slab of polyacrylamide sodium dodecyl sulfate gel. The extracted protein was then eluted from the hydroxylapatite by washing with increasing concentrations of phosphate buffer. The recovered protein was electrophoretically homogeneous. We believe that this method can be generally useful in the purification, for study, of proteins identified on two-dimensional gels as putative markers of disease.

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